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Screen grabber pro record bind6/11/2023 ĭyussembayev K, Sambasivam P, Bar I, Brownlie JC, Shiddiky MJA, Ford R (2021) Biosensor technologies for early detection and quantification of plant pathogens. ĭreischhoff S, Das IS, Häffner F, Wolf AM, Polle A, Kasper KH (2023) Fast and easy bioassay for the necrotizing fungus Botrytis cinerea on poplar leaves. ĭonoso A, Valenzuela S (2018) In-field molecular diagnosis of plant pathogens: recent trends and future perspectives. ĭean R, Van KJAL, Pretorius ZA, Hammond-Kosack KE, Di PA, Spanu PD, Rudd JJ, Dickman M, Kahmann R, Ellis J, Foster GD (2012) The top 10 fungal pathogens in molecular plant pathology. Front Microbiol 14(10):1889Ĭhen S, Yuan H, Yan X (2022) Rapid visual detection of benzimidazole resistance in Botrytis cinerea by recombinase polymerase amplification combined with a lateral flow dipstick. FASEB J Official Publication Federation Am Soc Exp Biol, 24(11), 4187–4195īilkiss M, Shiddiky MJA, Ford R (2019) Advanced diagnostic approaches for necrotrophic fungal pathogens of temperate legumes with a focus on Botrytis spp. Selection and versatile application of virus-specific aptamers. Int Immunopharmacol 67:260–267īalogh Z, Lautner G, Bardóczy V, Komorowska B, Gyurcsányi RE, Mészáros T (2010). Phytopathology, Jan 3.Īn JN, Ding SS, Hu XH, Sun LL, Gu YZ, Xu YY, Hu YM, Liu CP, Zhang XG (2019) Preparation, characterization and application of anti-human OX40 ligand (OX40L) McAbs and establishment of a sandwich ELISA for autoimmune diseases detection. Cross resistance of SDHIs in Botrytis cinerea and development of molecular diagnostic tools for SDHI resistance detection. The use of test strips enabled the detection of disease at a stage 4 days earlier than the traditional method.Īlzohairy SA, Heger L, Nik Zainal Alam N, Miles TD (2023). The results could be read directly within 15 min. The design was user-friendly, and all the steps required to perform the detection were sample preparation and sample loading. The strips could be conveniently stored in a dry environment (≤ 4 ☌) for > 6 months without any noticeable decrease in performance. The test strip prepared with antibodies against BcE4 reached a detection sensitivity of 6.25 μg/mL. The McAbs from BcA4 could bind a 25 kDa protein, while the antibodies from BcE4 could bind two proteins (53 and 340 kDa). cinerea from hybridoma cell lines BcA4 and BcE4, respectively. Results showed that we successfully obtained two highly specific McAbs, IgG3 and IgG2a, against B. After analyzing the titer, sensitivity, specificity, type, and binding protein of the prepared McAbs, we developed a colloidal carbon competitive immunochromatographic test strip with the McAbs. In this study, we prepared a monoclonal antibody (McAb) against a pathogenic fungus using classic hybridoma technology. Sensitive and rapid detection of fungal infections is highly desired as a preventive step to fight against the disease. The so-called “gray mold disease” of the plant that may lead to fatal economic loss is caused by the fungal pathogen Botrytis cinerea. As one of the most frequently used components of traditional Chinese herbal medicine, the economic plant Fritillaria thunbergii Miq is widely grown in several provinces of China.
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